Figure 1

Tau is recruited in SGs in neuronal cells. (a–d) HT22 cells were treated with 5 µM MG-132 for 4 h and stained with anti-Tau (TAU-5), anti-TIA1, anti-USP10, or anti-G3BP. Nuclei were stained with Hoechst in (c). Staining was visualized by a fluorescent microscope. Arrowheads indicate the colocalization of Tau with both TIA1 and USP10 and Tau with G3BP. SG-positive cells were counted as described in the Materials and Methods section, and the population (%) of SG-positive cells is presented as the mean and SD from three independent experiments in (b, d). **P < 0.01; ***P < 0.001. Scale bar: 10 µm. (e,f) Rat primary neuron-enriched cells were treated with 2 µM MG-132 for 8 h, and cells were stained with anti-Tau (TAU-5), anti-TIA1 and anti-USP10. SG-positive cells were counted, and the population (%) of SG-positive cells is presented as the mean and SD from three independent experiments in (f). *P < 0.05.