Figure 2
From: USP10 is a critical factor for Tau-positive stress granule formation in neuronal cells
USP10-knockdown reduces Tau/TIA1-SG in HT22 cells. (a) Knockdown of USP10 (USP10-KD) in HT22 cells was performed using the lentivirus vector encoding USP10/shRNA (USP10-3, USP10-4) or the control (NT). The expression of USP10, Tau, TIA1 and β-actin in these cells was measured by Western blotting using respective antibodies. The asterisk indicates a nonspecific band. (b,c) USP10-KD HT22 cells and the control (NT) were treated with 5 µM MG-132 for 4 h, and SG formation was evaluated by anti-Tau (TAU-5) and anti-TIA1 antibodies as well as Hoechst for nuclear staining using a fluorescent microscope. Arrowheads indicate the colocalization of Tau with TIA1. The population (%) of cells with TIA1-SGs or TIA1/Tau-SGs is presented as the mean and SD from three independent experiments in (c). *P < 0.05; **P < 0.01. Scale bar: 10 µm.
