Figure 4
From: USP10 is a critical factor for Tau-positive stress granule formation in neuronal cells
The deubiquitinase activity of USP10 is dispensable for Tau-SG formation. (a) A schematic illustration of USP10 and its mutants. (b–d) HT22 cells were transfected with a plasmid encoding HA-USP10 or its mutants (HA-USP10C424A, HA-USP101–274, HA- USP10275–798) by lipofection using Lipofectamine 2000. At 48 h after transfection, SG formation was evaluated by anti-Tau (TAU-5), anti-TIA1 and anti-HA antibodies using a fluorescent microscope. Arrowheads indicate the colocalization of Tau, TIA1 and USP10. The population (%) of cells with Tau/TIA1/USP10-SGs is presented as the mean and SD from three experiments in (d). **P < 0.01; ***P < 0.001; ****P < 0.0001. Scale bar: 10 µm. (c) The expression of USP10 and its mutants, Tau and β-actin in these cells was measured by Western blotting using anti-HA, anti-Tau (TAU-5) and anti-β-actin antibodies, respectively.
