Figure 5

MG-132 treatment disrupts the microtubule structure in HT22 cells. (a–d) HT22 cells were transfected with the HA-USP10 plasmid or control plasmid (HA) by the lipofection method using Lipofectamine 2000. At 48 h after transfection, cells were treated with 5 µM MG-132 or DMSO for 4 h. The localization of Tau and α-tubulin was evaluated by anti-Tau (TAU-5) and anti-α-tubulin antibodies as well as Hoechst using a fluorescence microscope. Arrows and arrowheads indicate Tau/α-tubulin aggregates and Tau aggregates, respectively. Cells with either Tau aggregates, α-tubulin aggregates or Tau/α-tubulin aggregates were counted, and the respective population (%) is presented as the mean and SD from three independent experiments in (b–d). **P < 0.01. NS indicates that a result is not statistically significant. Scale bar: 10 µm.