Figure 5
Expression analysis of fusion genes under reducing and non-reducing SDS-PAGE. (A) SDS-PAGE analysis of expression under reducing conditions and (B) under non-reducing conditions. Lane M: protein size marker, Lane 1: Total secretory proteins of GS115 transformed with pPICZαA plasmid, Lane 2: total secretory proteins of GS115 expressing IFNα2b-NS3-LAP fusion protein, Lane 3: total secretory proteins of GS115 showing expression of LAP-NS3-IFNα2b fusion protein. The bands of size 46.6 kDa indicate monomeric form of fusion proteins and 93.2 kDa bands indicate homodimeric form of fusion proteins.
