Figure 4 | Scientific Reports

Figure 4

From: Parvalbumin expression in oligodendrocyte-like CG4 cells causes a reduction in mitochondrial volume, attenuation in reactive oxygen species production and a decrease in cell processes’ length and branching

Figure 4

PV upregulation alters the morphology and soma mitochondria volume of CG4 cells. Cells were loaded with MitoTrackerRed CMXRos (Red), Hoechst (blue) and Calcein-AM (green) and z-stacks were imaged by confocal laser microscopy (A,H) to determine volumes of soma mitochondria, nuclei and soma cytoplasm, respectively. Quantification of total cell (soma) volume (B,I), cytoplasmic (soma) volume (C,J), volume of nucleus (D,K), ratio of nucleus/soma cytoplasm (E,L), soma mitochondria volume (F,M) and ratio of soma mitochondria/ soma cytoplasm (G,N) was performed by the Imaris software. The soma mitochondria volume and moreover the ratio of mitochondria/cytoplasm were significantly lower in PV-CG4 cells when compared to C-CG4 (control) and GFP-CG4 cells. Error bars represent SEM. (ns: not significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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