Figure 1 | Scientific Reports

Figure 1

From: Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model

Figure 1

Cardiomyogenic differentiation of murine iPSCs, elimination of undifferentiated iPSCs, and innate immune responses after transplantation of syngeneic iPSC-CMs in vivo. (A) CM-differentiation protocol. (B) Embryoid body of iPSC-CMs at day 16. Scale bar, 100 μm. (C) iPSC-CMs at day 16, stained with an anti-troponin T antibody (Alexa Fluor 488), an anti-phalloidin antibody (Alexa Flour 555), and 4′,6-diamidino-2-phenylindole (DAPI). Scale bar, 50 μm. (D) Flow cytometry data for iPSC-CMs at day 16, stained with anti-troponin T antibodies or an isotype-matched control. (E) Flow cytometry data for undifferentiated iPSCs at day 0 and (F) iPSC-CMs before and after cell sorting at day 16, stained with SSEA-1 antibodies or the isotype control. (G) Subcutaneous tissue 4 days after iPSC-CM syngeneic transplantation stained with anti-CD3 (Alexa Fluor 488), anti-CD68 (Alexa Fluor 488), anti-myeloperoxidase (Alexa Flour 647) and anti-CD335 (Alexa Flour 555) antibodies, and DAPI. Scale bars: 50 μm.

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