Table 2 Empirical amplification rates in dependence on the number of primer-template mismatches and other properties.

0

[0, 0]

[−16.616, −15.696]

100%

Overall

1

[0, 3]

[−14.353, −12.1]

100%

Overall

2

[0, 3]

[−12.0455, −9.656]

100%

Overall

3

[0, 4]

[−11.607, −7.9185]

100%

Overall

4

[2, 6]

[−10.796, −7.409]

92.31%

Overall

5

[0, 3]

[−7.047, −6.047]

88.89%

Overall

6

[0, 0]

[−8.603, −5.11325]

83.33%

Overall

7

[0, 3]

[−5.39, −4.212]

67.19%

Overall

8

[3, 6]

[−5.56075, −2.539]

34.04%

Overall

9

[4, 6]

[−3.5335, −2.1325]

23.08%

Overall

10

[4, 6]

[−4.09, −1.724]

18.02%

Overall

11

[4, 6]

[−3.74, −1.695]

10.53%

Overall

12

[6, 6]

[−2.624, −1.413]

3.75%

Overall

0

[0, 0]

[−16.07, −15.609]

100%

Set 1

1

[0, 3]

[−13.283, −12.1]

100%

Set 1

2

[0, 3.25]

[−11.94175, −9.656]

100%

Set 1

3

[0, 4]

[−11.607, −7.66375]

100%

Set 1

4

[2, 6]

[−10.974, −6.686]

90.91%

Set 1

5

[2.5, 4.5]

[−8.36825, −6.4925]

75%

Set 1

6

[3.25, 4]

[−4.4545, −2.9]

33.33%

Set 1

7

[3, 6]

[−4.212, −2.539]

9.52%

Set 1

8

[4, 6]

[−3.303, −2.06275]

18.06%

Set 1

9

[5, 6]

[−3.0985, −2.0395]

13.51%

Set 1

10

[5, 6]

[−3.393, −1.695]

11.26%

Set 1

11

[5, 6]

[−3.351, −1.695]

4.2%

Set 1

12

[6, 6]

[−2.608, −1.413]

2.6%

Set 1

0

[0, 0]

[−20.79275, −16.616]

100%

Set 2

1

[0, 2]

[−17.782, −14.045]

100%

Set 2

2

[0, 0]

[−14.4805, −12.5605]

100%

Set 2

3

[1, 1]

[−10.505, −10.505]

100%

Set 2

4

[0.75, 2.25]

[−10.29475, −9.29225]

100%

Set 2

5

[0, 0]

[−6.047, −6.047]

100%

Set 2

6

[0, 0]

[−8.603, −5.208]

100%

Set 2

7

[0, 0]

[−5.39, −5.208]

95.35%

Set 2

8

[0, 0]

[−5.937, −3.95]

86.36%

Set 2

9

[1, 6]

[−5.58, −2.89]

78.95%

Set 2

10

[0, 3]

[−5.208, −2.956]

66.67%

Set 2

11

[0, 2.25]

[−5.208, −2.8395]

64.29%

Set 2

12

[4, 5.5]

[−2.6225, −1.9615]

33.33%

Set 2