Figure 2
From: Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin
Comparison of CD (a) and absorption (b) spectra between two nanodiscs. The inset of panel a shows the nanodisc bands isolated by sucrose density gradient centrifugation. In panel b, the spectrum of DDM-solubilized GR is also shown. Here, all three spectra were plotted after subtracting the scattering contribution as shown in the inset, which shows the raw spectrum of the DDM-solubilized state and the estimated scattering artifact (for details, see Materials and Methods section). The buffer solution was 6-mix buffer, pH 7, containing 0.3 M NaCl. For DDM-solubilized GR, the medium was supplemented with 0.05% DDM.
