Figure 2

C. elegans motility recovers after 10 s of 20-MHz, 500 mW SAW exposure to become statistically indistinguishable with unexposed (control) worms in about one hour. Crowding (see text) was partially responsible for the initial reduction in motility for the control and 500-mW SAW-exposed worms, but a significant reduction in motility was observed due to SAW exposure alone. Mean (S.E.) values were obtained from N = 4 independent experiments, with n = 150–200 worms tested in each experiment (p < 0.05). Significance was based upon a two-way ANOVA over the n individual worm data and all N independent experiments for each reported time with \(\alpha =0.05\), where ns implies p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.0005. Normality of the data was assessed using the Shapiro-Wilk test with *p > α indicating the experimental data was normally distributed.