Figure 2
From: Transformation of normal cells by aberrant activation of YAP via cMyc with TEAD
Implication of contribution of TEAD, but not AKT, to YAP activity. (a) Expression levels of miR-29 genes in control NIH3T3 cells and cells expressing YAP or mtYAP under confluent conditions. Expression levels of miR-29a, miR-29b and miR-29c genes were normalized to those of U6 snoRNA. The expression level of each miRNA in control NIH3T3 cells was arbitrarily set to one. Levels of significance were examined by one-way ANOVA. (b) Western blot analyses of the indicated proteins in control NIH3T3 cells and cells expressing YAP or mtYAP under confluent conditions. The graph demonstrates the relative PTEN protein levels normalized using ActB protein levels as an internal control. (c) Immunocytochemical analyses of YAP in control NIH3T3 cells and cells expressing YAP or mtYAP under confluent conditions. (d) Expression levels of Ctgf and Serpine 1 in control NIH3T3 cells and cells expressing YAP or mtYAP under subconfluent (2 days after plating, d2) or confluent conditions (4 days after plating, d4). The expression level of each gene in control NIH3T3 cells was arbitrarily set to one. Data represent mean ± SD from three independent experiments. Levels of significance were examined by one-way ANOVA.
