Figure 5

Combinational analysis of HCC biomarkers enhanced the diagnostic accuracy. (A) Pearson analysis of correlations between anti-EIF3A autoantibody biomarker and AFP or anti-SF3B1 autoantibody was performed. (B) Combinational analysis of HCC biomarkers, anti-EIF3A autoantibody, AFP, or anti-SF3B1 autoantibody. The diagnostic values of each biomarker shown in Fig. 4A (anti-EIF3A and AFP) and Fig. 5A (anti-SF3B1) were simplified as either responsive (+) or non-responsive (−) according to whether their detection values were above or below the cutoff value. Then, we analyzed the diagnostic values of each biomarker or their combination: for the combined analysis of these markers, we simply added the unified diagnostic indexes of a serum sample and designated triple negative as -, single positive as in, double positive as ++, and triple positive as +++. Numbers on plots represent the number of corresponding subjects. (C) ROC curve analysis of combined biomarker tests with unified index shown in Fig. 5B. AFP and anti-SF3B1 autoantibody test with unified index were shown in Supplementary Fig S6 and the results of their ROC curve analysis were also plotted (left panels). AUC values of each ROC curve analysis were plotted. Sensitivity and accuracy of each test were also plotted (right panels) and these values were shown in Table 3.