Figure 3 | Scientific Reports

Figure 3

From: The genome sequence of Streptomyces rochei 7434AN4, which carries a linear chromosome and three characteristic linear plasmids

Figure 3

Terminal inverted repeat (TIR) of the S. rochei chromosome. (a) Nucleotide sequence comparison of inside ends of the TIR regions. Identical sequences are indicated by asterisks. Ch-L, chromosome left region; Ch-R, chromosome right region. (b) Southern hybridization analysis of the TIR regions. PCR fragment harboring nt 52,079–55,053 was used as a DNA probe to distinguish the TIR boundary. λ-DNA digested with HindIII was used as a DNA size marker. Lane M, λ/HindIII marker; lane 1, S. rochei 7434AN4 total DNA digested with BamHI; lane 2, S. rochei 7434AN4 total DNA digested with XhoI. (c) Restriction map of the left and right TIR regions of the S. rochei 7434AN4 chromosome. TIR regions of the chromosome are shown by thick black lines. Terminal proteins attached to the 5′-ends are indicated by filled circles. Some important restriction sites are indicated. Bg, BglII; Ba, BamHI; Xh, XhoI; Ec, EcoRI; Kp, KpnI.

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