Figure 7
From: Role of the kidneys in the redistribution of heme-derived iron during neonatal hemolysis in mice
Changes in H-ferritin (H-Ft) protein level and the absence of iron deposits in the kidneys of mice during the neonatal period. (a) Levels of H-Ft assessed in renal cytosolic extracts by Western blotting. The blot was reprobed with monoclonal anti-actin antibody as a loading control. (b) Immunolabeled H-Ft and actin control bands were quantified using a Molecular Imager and the relative levels of H-Ft (means ± S.D.) are plotted in arbitrary units (a.u). Results come from 2 separate blots and relative levels of proteins were standardized to a percentage with %S.D. in order to eliminate technical differences between blots. N values for each group are: 3dpp = 5, 5dpp = 5, 7dpp = 5, 9dpp = 5, 11dpp = 4. Data set for H-Ft has normal distribution, therefore, one-way ANOVA was used (p = 0,0014, df = 4, F = 6,849). Tukey’s Multiple Comparison Test was used as post-hoc test. Capital letters over the bars in the chart denote significant differences between age groups, with p < 0.01. (c) Histological examination of iron loading in the kidneys of mouse neonates. No non-heme iron deposits were detected by staining with Perls’ Prussian Blue. Kidney section from 250-day-old HO1 knock-out mouse was used as a positive control of iron loading in renal cortex (indicated by arrows) during excessive hemolysis37. dpp – days postpartum.
