Figure 5
From: Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells
Electrophysiological properties of dorsal (A–D) and ventral (E–H) spheroids (day 45). (A,E) Representative voltage clamp traces showing fast inward Na+ currents and long-lasting outward K+ currents evoked by depolarizing voltage steps. Step size = 15 mV. The inset shows a high magnification view of the inward currents. (B,F) Representative current clamp traces showing rebound action potentials in response to hyperpolarizing current injections. Step size = 20 pA. The inset shows a high magnification view of the rebound action potentials. (C,G) Representative traces of continuous voltage clamp recording showing spontaneous postsynaptic currents. The inset shows a high magnification view of one spontaneous postsynaptic current. (D,H) Representative phase-contrast images of a recorded neuron. Scale bar: 20 μm.
