Figure 7
From: Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells
Co-Culture of microglia-like cells with ventral, dorsal or ventral/dorsal organoids: immunomodulatory properties. Co-cultured organoids (day 35) were stimulated with Aβ42 oligomers for 72 hours before the analysis. (A) RT-PCR analysis of (i) TNF-α and (ii) IL-6 gene expression (n = 3). (B) The secretion of cytokines (i) VEGF-A and (ii) PGE2 (n = 3) determined by ELISA assay. (C) Effect of NF-kB inhibitor BAY11–7082 (10 μM) on mRNA expression of TNF-α determined by RT-PCR (n = 3). (i) MGs and (ii) Co-cultured spheroids/organoids treated with BAY11-7082 in the presence of Aβ42 oligomers; Dash line represents the dorsal NPC only control. (D) Effect of NF-kB inhibitor BAY11-7082 (10 μM) on mRNA expression of TREM2 determined by RT-PCR (n = 3). (i) MGs and (ii) co-cultured spheroids/organoids treated with BAY11-7082 in the presence of Aβ42 oligomers. Dash line represents the dorsal NPC only control. *Indicates p < 0.05 for the different test conditions.
