Figure 6
From: X-ray Crystallographic Structure and Oligomerization of Gloeobacter Rhodopsin
EPR analysis of the oligomeric states of spin labeled GR-67R1. (a) Spin-labeling site on GR viewed parallel to the membrane (left) and from the cytoplasmic side (right). The α-carbon of Gly67 is colored magenta and shown as a sphere. (b–d) Superimposed structure of GR and HsBR (b) GR and KR2 (c) and BPR (d). α-carbon of the residues equivalent to Gly67 in GR are shown as a sphere. (e) Room temperature CW EPR spectra of GR-67R1 in 0.05% DDM at pH 3.0 and pH 8.0. Dipolar broadening in the pH 8.0 spectrum is clearly observed. (f) DEER distance distribution of GR-67R1 at pH 8.0. Inset: baseline-corrected DEER traces.
