Figure 1 | Scientific Reports

Figure 1

From: Unraveling the role of quorum sensing-dependent metabolic homeostasis of the activated methyl cycle in a cooperative population of Burkholderia glumae

Figure 1

Activation of S-adenosylmethionine (SAM)-dependent methyltransferase and ahcY genes by quorum-sensing (QS) in B. glumae. (A) Expression levels of SAM-dependent methyltransferase genes and (B) ahcY in B. glumae BGR1 (wild type), BGS2 (BGR1 tofI::Ω), and BGS9 (BGR1 qsmR::Ω). Gene expression levels were quantified by quantitative real-time PCR (qRT-PCR) after 10 h of incubation with three biological replicates. The letters (a, b, and c) above each mean represent statistical significance based on ANOVA/Tukey’s correction for multiple comparisons. A value of p < 0.05 represents a significant difference among strains. (C) Binding of QsmR-His to the putative promoter region of ahcY. Gel mobility shift assays show that purified QsmR-His binds to the DNA fragment carrying putative promoter region of ahcY.

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