Figure 3 | Scientific Reports

Figure 3

From: Modular microfluidic systems cast from 3D-printed molds for imaging leukocyte adherence to differentially treated endothelial cultures

Figure 3

Production of the barrier module for seeding of two endothelial cell populations separated by a 250 µm barrier on a glass slide. (a) The barrier module was cast in PDMS from a 3D-printed mold. (b) Precise positioning of the barrier module to a glass slide using a 3D-printed alignment tool and attached using vacuum. (c) The barrier module vacuum-attached to a glass slide with its cell seeding channels filled with either a red or a green dye. (d) Endothelial cell populations grown in the barrier module overnight and thereafter stained for 4 h in their respective channels with Celltracker green or Celltracker red dyes, and imaged using a fluorescence microscope.

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