Table 6 Specific PCR Primer sequence and reaction conditions of MT-ND2 (Product size ≈ 1000 bp), MT-ND5 (≈347 bp), MT-ATP6 (≈673 bp), MT-ATP8 (≈110 bp) and MT-CYB (≈523 bp).
Gene | Primers (bp) | Product | Annealing temp. | No. of cycles |
|---|---|---|---|---|
MT-ND2 | 5′ATTAATCCCCTGGCCCAACC3′ | 1000 | 48 | 30 |
5′TGGTAAGGGCGATGAGTGTG3′ | ||||
MT-ND5 | 5′CGGAAGCCTATTCGCAGGAT3′ | 347 | 48 | 28 |
5′TGGAGGTGGAGATTTGGTGC3′ | ||||
MT-ATP6 | 5′CTCACCAAAGCCCATAAA3′ | 673 | 52 | 35 |
5′AGGCGACAGCGATTTCTA3′ | ||||
MT-ATP8 5′ACTACCACCTACCTCCCTCAC3′ | 110 | 49 | 30 | |
5′GGATTGTGGGGGCAATGAATG3′ | ||||
MT-CYB | 5′GGGACAGACCTAGTTCAATG3′ | 523 | 55 | 35 |
5′CTGCGGCTAGGAGTCAATAA3′ |
