Figure 5
From: Neuroprotection mediated by cystatin C-loaded extracellular vesicles
CysC enhances EV levels in the brain extracellular space of CysC transgenic mice but does not specifically regulate ESCRT-dependent exosome secretion. EVs were isolated from hemibrains of 12-month-old TgCysC and wild-type (WT) littermate control mice. EV protein content (a), EV AChE activity (b) and the number of particles in the EV brain preparations assessed by NTA as described in the Methods (c) were standardized relative to protein amount in the hemibrain. Representative Western blots of the sucrose-gradient fractions b, c and d containing EVs (d) and the corresponding quantification (e) showing the levels for the EV markers Alix, TSG101, CD9, Flotillin-1, and Flotillin-2 in EVs isolated from the brains of TgCysC and WT mice. Elevated CysC levels in TgCysC EVs due to the CysC overexpression in the transgenic mice are also shown. All data are shown as the TgCysC to control ratio and are presented as mean and SEM (*p < 0.05; **p < 0.01).
