Table 1 Enzyme activities of GalE enzymes.

bGalE

—

158

147

—

Specific activity (U/mg) at 1 mM UDP-sugar

¹⁵

hGalE

—

33.8

—

26

Specific activity (U/mg) at 0.41 mM UDP-Gal or 0.66 mM UDP-GalNAc

²⁷

hGalE

(C307Y)

1.21

0.02

Relative activity compared with the wild type enzyme at 2 mM UDP-Gal or 1.89 mM UDP-GalNAc

⁴³

eGalE

—

23.9

—

0.003

Specific activity (U/mg) at 0.41 mM UDP-Gal or 0.66 mM UDP-GalNAc

²⁷

eGalE

(Y299C)

—

5.1

—

0.69

tGalE

—

5.9

—

—

Specific activity (U/mg) at 20 mM UDP-Gal

⁵⁴

WbpP

0.124

0.188

120

271

k_cat (min⁻¹). K_m values were similar for all substrates (197~251 uM)

⁵⁵