Figure 1
Phylogenetic analysis of Ag43 passengers and their subdomains. The amino acid sequences of the passengers were aligned using T-Coffee and structural information of Ag43C3-UPI00000E4A66 (PDB: 4KH3) as template. Based on the L-shaped Ag43 structure (Fig. 2), the amino acid sequences of the SL, EJ and BL subdomains were recovered for alignment. Trees were generated using SplitsTree applying bootstrapping and 95% confidence for the observed nodes. Inserts represent the trees before applying bootstrapping and confidence threshold as provided in Supplementary Fig. S1. (A) Tree of the Ag43 passengers highlighting the four classes, C1, C2, C3 and C4. (B) Tree of the SL subdomain highlighting the two subtypes, SL1 and SL2. (C) Tree of the EJ subdomain highlighting the two subtypes, EJ1 and EJ2. (D) Tree of the BL subdomain highlighting the two subtypes, BL1 and BL2. For clarity, some sequences were removed from the trees but are readily available in Supplementary Table S1 and Fig. S1. The scale bars represent the number of substitutions per site.
