Figure 3
Autoaggregation of E. coli SRUD expressing different Ag43 variants. (A) The sedimentation assay was performed in LB at 37 °C over 6 h with SRUD expressing variants of Ag43 from C1 (shades of green), Ag43C2 (shades of grey), (iii) Ag43C3 (shades of red), Ag43C4 (shades of blue). The nonaggregative SRUD pBAD was used as negative control. (B) Sedimentation was visualised after 6 h of static incubation. (C) Phase-contrast microscopy observation from sampling at 6 h of static incubation. PT: prototypical variant (Table 1).
