Figure 4

Fluorescent microscopy analysis of homotypic interactions of bacterial cells expressing different variants of Ag43. Epifluorescence microscopic observations were performed from bacterial cells grown in LB and after 6 h of static incubation at 37 °C. Co-culture of nonaggregative (NA) SRUD-Red pBAD with (A) SRUD-Green pBAD, (B) SRUD-Green Ag43^C1-PT, (C) SRUD-Green Ag43^C2-PT, (D) SRUD-Green Ag43^C3-PT, or (E) SRUD-Green Ag43^C4-PT. Co-culture of SRUD-Red and SRUD-Green both expressing (F) Ag43^C1-PT, (G) Ag43^C2-PT, (H) Ag43^C3-PT, or (I) Ag43^C4-PT. The NA, C1, C2, C3 and C4 labels match the colour of the fluorescent bacterial strains.