Figure 7

Characterisation of heterotypic interactions of Ag43 variants by flow cytometry. Flow cytometry analyses were performed by mixing SRUD-Green and SRUD-Blue expressing respectively Ag43^C1-PT (C1) and Ag43^C2-PT (C2), Ag43^C2-PT and Ag43^C4-PT (C4), Ag43^C1-PT and Ag43^C3-PT (C3), Ag43^C2-PT and Ag43^C3-PT, Ag43^C1-PT and Ag43^C4-PT, or Ag43^C3-PT and Ag43^C4-PT. (A) Based on the SSC-A over FSC-A representation (pictured in the insert), the fluorescent FITC-H over PB450-H representation focused on the gate corresponding to the cell aggregates as previously defined (Supplementary Fig. S3). Graphs correspond to one representative experiment. (B) The percentage of mixed cell aggregates was calculated from the events detected in orange cloud over the total number of events corresponding to bacterial cell aggregates. (C) The concentration of single individual cells was calculated from the corresponding gate as previously defined (Supplementary Fig. S3). (D) The size of the aggregates was recovered from the gate for cell aggregates as previously defined (Supplementary Fig. S3).