Figure 3 | Scientific Reports

Figure 3

From: A protein scaffold, engineered SPINK2, for generation of inhibitors with high affinity and specificity against target proteases

Figure 3

The inhibitory activities of chymotrypsin, KLK1, KLK4, and KLK8 binders. Enzymatic assays of (a) chymotrypsin, (b) KLK1, and (c) KLK4 with selected specific inhibitors, and of (d) KLK4 and (e) KLK8 with KLK4/8 dual inhibitors. Three independent experiments were performed, and one of three was shown as figures. (f) IC50 values determined from three independent experiments by sigmoidal nonlinear regression logistic four parameter analysis using GraphPad Prism 5.0 (GraphPad software).

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