Figure 3 | Scientific Reports

Figure 3

From: The interleukin-33-mediated inhibition of expression of two key genes implicated in atherosclerosis in human macrophages requires MAP kinase, phosphoinositide 3-kinase and nuclear factor-κB signaling pathways

Figure 3

JNK1/2 is required for the IL-33-mediated inhibition of MCP-1 and ICAM-1 expression in human macrophages. Knockdown using siRNA against JNK1/2 (JNK) or negative control sequence (Neg) was carried out as Materials and Methods. THP-1 macrophages were then incubated for 12 h in the presence of vehicle (−) or 25 ng/ml IL-33 (+). The mRNA expression of JNK1 (A), JNK2 (B), MCP-1 (D) and ICAM-1 (E) was analyzed by RT-qPCR. Data represents mean ± SEM from five independent experiments. The knockdown of JNK1 (A) or JNK2 (B) in vehicle- or IL-33 treated cells was determined in relation to the negative control, which was arbitrarily assigned as 1 (A,B). The IL-33-mediated changes in MCP-1 and ICAM-1 expression in the negative control was compared to that following knockdown of JNK1/2 (D,E) with values from cells transfected with negative control siRNA or JNK1/2 siRNA and then treated with vehicle alone given an arbitrary value of 1. Statistical analysis was carried out using an unpaired Student’s t-test (*p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001). The expression of JNK1/2 protein levels was determined by Western blot analysis using β-actin as a control (C). A representative image from two independent experiments with signals from immunoreactive JNK1/2 or β-actin is shown (see Supplementary Fig. 5 for corresponding full-length image).

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