Figure 3
From: Photothermal incubation of red blood cells by laser for rapid pre-transfusion blood group typing
Laser light absorption by RBCs. (a) Heating block incubation heats water, whole and lysed RBC suspensions (75 µL) at the same rate, reaching 37 °C in 150 seconds. (b,c) Laser illumination rapidly heats sample volumes (75 µL), reaching 37 °C in under 30 seconds. (b) Laser illumination is pulsed on (grey) and off (white) to heat and maintain sample temperature between 36 °C and 38 °C. (c) Without pulsing, the sample will heat to 70 °C. (d) Increasing the concentration of both whole and lysed RBCs increases the heating rate. (e) Absorption spectra for RBCs and water across the visible and near-infrared wavelength ranges. (f) Absorption of lysed RBC suspensions for various concentrations around 980 nm (water baseline absorption subtracted).
