Figure 4
From: Gene-environment interaction counterbalances social impairment in mouse models of autism
Abnormal mEPSC amplitudes in Cntnap2 KO and VPA-exposed mice are normalized by the interaction of these factors. Whole-cell patch-clamp recordings in neurons in layer 2/3 of the mPFC. (a) Representative mEPSC traces. (b) mEPSC amplitudes. (c) mEPSC frequencies. Two-way ANOVA followed by Fisher’s LSD test was performed to determine between-group differences. WT × Veh, n = 7; KO × Veh, n = 7; WT × VPA, n = 19; KO × VPA, n = 16. In (b) asterisk (*) shows the results of statistical comparison between WT × Veh and the indicated group; *, p < 0.05. Sharp (#) shows the results of statistical comparison between the indicated groups; #, p < 0.05, ##, p < 0.01, ###, p < 0.001. In all bar graphs, data are the mean ± S.E.M. Abbreviations: WT, Cntnap2 WT mice; KO, Cntnap2 KO mice; VPA, treatment with valproic acid (300 mpk) at E10.5; Veh, treatment with phosphate-buffered saline at E10.5.
