Figure 4

Genomic and phenotypic characterization of the antimicrobial resistance capacity of Colombian Clostridium difficile clinical isolates. (a) Frequency of antimicrobial resistance molecular markers (AMR-MMs) identification in reads obtained from whole genome sequencing of the clinical isolates. (b) MIC50 against 10 antimicrobial agents MET, metronidazole; VAN, vancomycin; TET, tetracycline; ERY, erythromycin; RIF, rifampicin; AMP, ampicillin; PEN, penicillin; FUS.AC, fusidic acid; CLI, clindamycin; MOX, moxifloxacin. The red dotted line represents the average concentration evaluated for each antimicrobial agent. MLS: Macrolide-lincosamide-streptogramin. The graphical representation of results was developed in Plotly server66. (c) Comparison of the detection frequency of the AMR-MMs with the MIC50 results. We identified as an association those comparisons that had a p < 0.05 after a Chi2 test. *Concentration range for MET, RIF and MOX was from 0.39 to 13; for AC.FUS, AMP and PEN was from 0.78 to 25; for VAN and TET was from 4.69 to 150; for ERY was from 1.17 to 38; and for CLI was from 0.15 to 5.