Figure 5

Water retention capacity of the seedling root. (a) Representative images showing water retention of the root from control (Cont) and transgenic lines overexpressing RHS10 (RHS10ox), axr2-1 (axr2-1ox), RSL4 (RSL4ox), and IAA2mImI (IAA2mImIIox) at the start point (immediately after pulling out the root from the agar medium) and the end point (when retained water has disappeared). Bar is 500 µm for all. Red bars in RSL4ox images indicate the water film widths including the root itself, which represents the capacity of water retention by the root. (b) The dynamics of water retention after pulling out the root from the agar medium. As shown in the images for RSL4ox in a, the widest width of the water film retained by the root was measured every 15 s after pulling out the root. Data represent mean ± s.e.m. (n = 25 roots from each line). Statistically significant differences at time 0 are denoted with different letters (one-way ANOVA with Tukey’s unequal N-HSD post hoc test, P < 0.05).