Figure 5 | Scientific Reports

Figure 5

From: Involvement of the P2X7 receptor in the migration and metastasis of tamoxifen-resistant breast cancer: effects on small extracellular vesicles production

Figure 5

No involvement of EMT or MMP-2/9 expression in P2X7-mediated cell migration. (A) Expression of EMT markers (E-cadherin, N-cadherin, Vimentin, Zeb-1, and Snail) in MCF-7 and TAMR-MCF-7 cells after treatment with 3–100 μM ATP for 48 h. (B) Expression of EMT markers (E-cadherin, N-cadherin, Vimentin, Zeb-1, and Snail) in MCF-7 and TAMR-MCF-7 cells after treatment with 3 μM KN62 for 48 h. (C) Immunocytochemical stainings of DAPI, E-cadherin and phalloidin in MCF-7 and TAMR-MCF-7 cells (x 20). (D) Expression of MMP-2 and MMP-9 in MCF-7 and TAMR-MCF-7 cells after treatment with 10–100 μM ATP for 48 h. (E) Expression of MMP-2 and MMP-9 in MCF-7 and TAMR-MCF-7 cells after treatment with 3 μM KN62 for 48 h.

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