Figure 6

Role of P2X7 in sEV secretion in TAMR-MCF-7 cells. (A) Effect of KN62 on the production of sEVs in MCF-7 cells and TAMR-MCF-7 cells. The number sEVs was determined by nanoparticle tracking analysis after treatment of cells with 3 μM KN62 for 24 h (normalized by initial number of plating cells)(n = 3). (B) Effect of KN62 on the production of sEVs in T47D and TAMR-MCF-7 cells (n = 3). (C) Protein expression of CD63 and Rab5 in MCF-7 and TAMR-MCF-7 cells (n = 3). (D) Effects of KN62 on the protein expression of CD63 (n = 3) and Rab5 (n = 5) in MCF-7 and TAMR-MCF-7 cells. (E) Effects of KN62 on the protein expression of CD63 (n = 6) and Rab5 (n = 6) in MDA-MB-231 cells. (**p < 0.01, ***p < 0.005, significant as compared to MCF-7 cells; ^#p < 0.05, ^###p < 0.005, significant as compared to TAMR-MCF-7 control group; ⁺⁺p < 0.01, significant as compared to T47D control group).