Table 2 Overview of the cell types demonstrating VSBV-1 antigen by IHC in tissues from infected squirrels.

Central Nervous System

6/6*

7/8*

Neurons, glial cells, Purkinje cells, granule cells, ependymal cells

Peripheral Nervous System

6/6

7/7

Nerve fibres, peri- and endoneurium, neurons of ganglia

Nose

5/6

5/7

Olfactory and respiratory epithelium, glands, nerve cells, connective tissue

Lung

6/6

5/8

Bronchial epithelium, alveolar walls, alveolar macrophages, type 2 pneumocytes, BALT, connective tissue, smooth muscle cells

Intestine

6/6

6/8

Goblet cells, epithelium, connective tissue and myocytes of lamina propria and muscularis mucosae, tela submucosa, tunica muscularis, ganglia

Liver

0/6

0/8

None

Salivary gland

1/5

5/7

Glandular cells, nerves, vessels

Kidney

4/6

5/8

(Mainly distal) tubular epithelium, Bowman’s capsule of glomeruli

Urinary Bladder

2/4

6/7

Epithelium, muscularis, glands, nerve cells

Ovary/Uterus

2/2

2/3

Connective tissue, muscle cells, uterine glands

Testes/Penis

3/4

2/5

Connective tissue, muscle cells, urethral epithelium, spermatozoa (and precursor cells)

Skin

6/6

7/8

Sebaceous glands, hair follicles, epithelium, sweat glands, nerve cells

Lacrimal gland

3/5

1/1

Glandular cells, connective tissue, nerves, vessels

Tonsil

1/2

5/7

Small round cells with minimal chromatin and a basophilic nucleus, oval to round cells with abundant chromatin and a large nucleus, interstitial cells, epithelium

Spleen

1/6

4/8

Small round cells with minimal chromatin and a basophilic nucleus, oval to round cells with abundant chromatin and a large nucleus, interstitial cells

Lymph nodes

0/4

2/3

Small round cells with minimal chromatin and a basophilic nucleus, oval to round cells with abundant chromatin and a large nucleus, interstitial cells