Figure 1

Copper isotopic composition of serum and brain in wild type mice. The data are reported as δ⁶⁵Cu, which is the per mil deviation of the ⁶⁵Cu/⁶³Cu from the NIST 976 standard. (A) Evolution of δ⁶⁵Cu in serum of individual mice are shown over time (males are shown in blue and female in red). (B) Pooled data for δ⁶⁵Cu in serum: boxes extend from the 25^th and 75^th percentile, the line in the middle of the box represents the median, and the whiskers show the minimum and the maximum. Two-way ANOVA showed a significant effect of sex (p < 0001). p values between males and females are indicated on the graphic for each age (Sidak’s multiple comparison test). Wilcoxon matched-pairs rank test were used to compare females of different ages (p = 0.06 when comparing 3- and 12-month-old females). (C) Comparison of δ⁶⁵Cu in serum and brain from 12-month-old females. p = 0.06, Wilcoxon matched-pairs rank test.