Figure 3

The effects of maternal transmission of Igf2r^I1565A/+p on embryonic and placental growth. (a) Comparison of heterozygote (Igf2r^I1565A/+p) and homozygote (Igf2r^{I1565A/I1565A}) embryo wet and dry weights following maternal transmission of Igf2r^I1565A/+p. PCR confirmed genotypes at E14.5, E17.5 and E18.5. Number of embryos per genotype and time-points are shown along the x-axis. Wet weight; E17.5 ***p < 0.0001, E18.5 **p = 0.0001. Dry weight; E17.5 **p = 0.007, E18.5 **p = 0.0038, NS p = 0.089. (b) Water content (WC = wet weight – dry weight) for data in (a). E17.5 **p = 0.012, E18.5 ***p = 0.0002. (c) Comparison of wet and dry weights of placentas following maternal transmission of Igf2r^I1565A/+p resulting in heterozygote and homozygote genotypes as in (a). Wet weight; E17.5 NS p = 0.0566, E18.5 **p = 0.0054. Dry weight; E17.5 NS p = 0.0620. (d) Water content for data in (c). E17.5 NS p = 0.1522, E18.5 **p = 0.0061. Horizontal line = mean, box plots 95% ± whiskers maximum and minimum. For statistical analysis, one-way ANOVA with Bonferroni multiple comparisons test. NS-not significant.