Table 4 Selected parameters from PISA evaluation of the dimerization interfaces in ANO5 and its mutants.

From: ANO5 mutations in the Polish limb girdle muscular dystrophy patients: Effects on the protein structure

Model

Chain A

Chain B

Interface A-B

N(at)

N(res)

Surface [Ẳ2]

N(at)

N(res)

Surface [Ẳ2]

Surface [Ẳ2]

ΔG [kcal/mol]

ΔG P-value

ANO5

279

70

42474

307

85

41873

2520.7

−39.7

0.142

p.Asp81Gly

358

94

48767

345

88

48092

3033.2

−36.2

0.661

p.Lys132Met

376

97

47313

381

97

48815

3314.8

−47.0

0.375

p.Ser555Ile

456

121

47717

433

110

49308

3887.3

−46.8

0.713

p.Tyr671Cys

375

105

40483

358

102

39668

3226.0

−48.9

0.202

p.Arg758Cys

360

90

46726

348

91

48777

2977.8

−27.8

0.906

p.His841Asp

320

90

47315

323

92

47046

2844.7

−42.6

0.243

  1. PISA, protein interfaces, surfaces and assemblies service at the European Bioinformatics Institute (http://www.ebi.ac.uk/pdbe/prot_int/pistart.html); N(at) and N(res) indicate the number of interfacing atoms and residues, respectively. ΔG and ΔG P-value indicate the solvation free energy gain and the P-value of the solvation free energy gain, respectively.
Back to article page