Figure 3 | Scientific Reports

Figure 3

From: The droplet size of emulsion adjuvants has significant impact on their potency, due to differences in immune cell-recruitment and -activation

Figure 3

Cell recruitment induced by selected adjuvants at different timepoints post-vaccination. Mice were immunized IM with fluorescent OVA-AF647 together with the adjuvants of interest. At various timepoints animals were euthanized and organs harvested for analysis of immune cell composition at SOI. Once the muscle cells were homogenized into a single cell suspension and stained with the antibody mixture, they were analyzed by multi-color flow cytometry. Number of animals per time-point were: 3 animals for 6 h and 72 h, and 6 animals for 24 h and 48 h.

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