Figure 4

Antigen positive immune cells at the dLNs of same mice shown in Fig. 3. Once the dLNs were homogenized into a single cell suspension and stained with the antibody mixture, they were analyzed by flow cytometry. Numbers of animals per time-point were: 3 animals for 6 h and 72 h, and 6 animals for 24 h and 48 h. Each animal was injected in both thighs; so each animal indicates two replicates since dLNs near both SOI were harvested.