Figure 1

Effects of IL-4 on HSL expression, stability and degradation (a) 3T3-L1 mature adipocytes were serum-free starved for 2 hr then treated with IL-4 (10 ng/mL) for the time indicated, then Hsl mRNA was analyzed by real-time PCR. Gene expression was normalized to 18 s rRNA (n = 7). (b) Hsl promoter activity under IL-4 treatment was analyzed by dual-luciferase reporter assay as detailed described in Methods. (c) Mature adipocytes were treated with IL-4 (10 ng/mL) or CHX (10 μg/mL) for the indicated time after 2 hr of serum starvation. The half-lives of HSL and perilipin were analyzed by Western blotting (n = 11). The data were shown by grouping of blots cropped from different parts of the same gel.