Figure 2

Dose and temporal effects of isoproterenol on IL-4-mediated phosphorylation of HSL, perilipin and PKA. (a) After 2 hr of serum starvation, adipocytes were pre-treated with 10 ng/mL IL-4 for 1 hr, followed by adding various concentrations of Iso for additional 1 hr. Then the phosphorylated protein levels were examined by Western blot. (b) Quantification of (a). (c) After 2 hr of serum starvation, adipocytes were treated with 10 μM Iso in the presence or absence of 10 ng/mL IL-4 for the time indicated. (d) Quantification of (c). *p < 0.05. The data were shown by grouping of blots cropped from different parts of the same gel.