Figure 11 | Scientific Reports

Figure 11

From: Ruthenium(II) complexes with 6-methyl-2-thiouracil selectively reduce cell proliferation, cause DNA double-strand break and trigger caspase-mediated apoptosis through JNK/p38 pathways in human acute promyelocytic leukemia cells

Figure 11

Effect of ruthenium(II) complexes with 6-methyl-2-thiouracil in phospho-JNK2 (T183/Y185), phospho-p38α (T180/Y182) and phospho-ERK1 (T202/Y204) expressions determined by phospho-specific ELISA in HL-60 cells treated with the complexes at 2 µM for 15 and 30 min incubation. (A) Quantification of phospho-JNK2 (T183/Y185) expression. (B) Quantification of phospho-p38α (T180/Y182) expression. (C) Quantification of phospho-ERK1 (T202/Y204) expression. Negative control (CTL) was treated with vehicle (0.2% DMSO) used for diluting the complexes, and doxorubicin (DOX, 1 µM) was used as positive control. Data are presented as mean ± S.E.M. of at least three independent experiments performed in duplicate. *p < 0.05 compared with negative control by ANOVA followed by Student Newman-Keuls test.

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