Figure 5 | Scientific Reports

Figure 5

From: Ruthenium(II) complexes with 6-methyl-2-thiouracil selectively reduce cell proliferation, cause DNA double-strand break and trigger caspase-mediated apoptosis through JNK/p38 pathways in human acute promyelocytic leukemia cells

Figure 5

Effect of ruthenium(II) complexes with 6-methyl-2-thiouracil in the morphological analysis of HL-60 cells, assessed by light scattering features using flow cytometry. Representative flow cytometry dot plots are presented in (A) and quantification of forward light scatter (FSC) and side-scattered light (SSC) after 12 h of treatment are presented in (B) and after 24 h of treatment in (C). FSC and SSC were measured as parameters of cell-surface area or size and cell granularity or internal complexity, respectively. Negative control (CTL) was treated with vehicle (0.2% DMSO) used for diluting the complexes, and doxorubicin (DOX, 1 µM) were used as positive control. Data are presented as mean ± S.E.M. of at least three independent experiments performed in duplicate. Ten thousand events were evaluated per experiment, and cellular debris was omitted from analysis. *p < 0.05 compared with the negative control by ANOVA followed by Student Newman-Keuls test.

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