Table 1 Cytotoxic activity of ruthenium(II) complexes with 6-methyl-2-thiouracil.

From: Ruthenium(II) complexes with 6-methyl-2-thiouracil selectively reduce cell proliferation, cause DNA double-strand break and trigger caspase-mediated apoptosis through JNK/p38 pathways in human acute promyelocytic leukemia cells

Cells

IC50 and 95% CI (µM)

DOX

OXA

(1)

(2)

Cancer cells

HL-60

0.02

0.02–0.06

0.40

0.03–3.75

0.01

0.01–0.52

0.13

0.03–0.61

K-562

0.06

0.04–0.08

0.90

0.08–9.90

0.20

0.10–0.40

0.34

0.17–0.73

HCT116

0.07

0.04–0.12

4.13

2.67–6.42

1.38

0.76–2.53

1.16

0.94–1.47

HepG2

0.02

0.02–0.08

0.96

0.23–3.94

0.95

0.44–2.07

0.83

0.26–2.65

SCC-4

1.43

1.02–2.02

6.98

3.28–14.94

1.63

0.82–3.27

1.21

0.78–1.90

HSC-3

0.25

0.18–0.36

6.80

4.73–9.75

4.37

2.65–7.24

2.04

1.72–2.46

B16-F10

0.02

0.02–0.04

0.03

0.02–0.05

0.83

0.60–1.16

1.04

0.74–1.47

Non-cancer cells

MRC-5

1.51

1.20–1.92

1.54

0.86–2.85

5.71

2.60–12.55

3.06

2.38–4.01

PBMC

5.17

2.56–10.43

14.88

8.91–24.83

2.57

2.07–3.18

1.25

0.67–2.32

  1. Data are presented as IC50 values and their respective 95% confidence interval (95% CI) in µM obtained by nonlinear regression from at least three independent experiments performed in duplicate, measured by alamar blue assay after 72 h of treatment. Cancer cells: HL-60 (human acute promyelocytic leukemia); K-562 (human chronic myelogenous leukemia); HCT116 (human colon carcinoma); HepG2 (human hepatocellular carcinoma); SCC-4 (human oral squamous cell carcinoma); HSC-3 (human oral squamous cell carcinoma); and B16-F10 (mouse melanoma). Non-cancer cells: MRC-5 (human lung fibroblast) and PBMC (human peripheral blood mononuclear cells). Doxorubicin (DOX) and oxaliplatin (OXA) were used as positive controls.
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