Figure 3
From: Cucumber mosaic virus-induced gene silencing in banana
CMV20-based VIGS of glutamate 1-semialdehyde aminotransferease (MaGSA) or phytoene desaturase (MaPDS) in banana. (a) Phenotype of banana inoculated with Agrobacterium containing pJL89 (mock), or Agrobacterium mixture carrying pJLCMV20-R1, pJLCMV20-R3 plus pJLCMV20-R2E (CMV20-R2E), pJLCMV20-35S (CMV20-35S), or pJLCMV20-MaGSA (CMV20-MaGSA) at 30 dpi. Bar = 5 cm. Quantification of the mRNA levels of CMV 20 coat protein and MaGSA was analyzed by quantitative RT-PCR, normalized relative to the level of actin. (b) Phenotypes of banana inoculated with Agrobacterium containing pJL89 (mock), CMV20-R2E, CMV20-35S, or pJLCMV20-MaPDS (CMV20-MaPDS) at 30 dpi. Bar = 5 cm. Quantification of the mRNA levels of CMV20 coat protein and MaPDS was analyzed by quantitative RT-PCR, normalized relative to the level of actin. Quantitative RT-PCR data represent mean ± SD; 3 representative technical repeats; ND, not detectable. Different letters indicate statistically significant differences analyzed by one-way analysis of variance (ANOVA) Tukey’s test (P < 0.05).). ****P < 0.001, Student’s t test compared to CMV20-35S agro-inoculum inoculated plants.
