Figure 5
Endothelial-specific deletion of IL-7Rα decreases lymphatic drainage and increases edema formation in imiquimod-induced inflammation. (a) Imiquimod-containing cream was applied to the ears of IL-7RαΔEC and control (CTR) mice on days 1–5, and 7. The ear thickness was measured on each treatment day. Lymphatic drainage was measured on day 7 after challenge and the mice were sacrificed on day 8. The immune cell infiltration in the ear skin was analyzed by FACS. (b) Ear thickness measurements over the course of the treatment. *p < 0.05; **p < 0.01. (c,d) Lymphatic drainage analysis: (c) Average clearance plots of P20D800 from the ear measured by IVIS and (d) the calculated half-lives. FACS-based quantification of the number of (e,i) CD45+ cells, (f) neutrophils, (g) CD3+ T cells, (h,l) DCs, (j) CD4+ T cells, (k) CD8+ T cells in (e–h) the ear skin and (i–l) dLNs on day 8. The average number of immune cells per ear is shown. Pooled data (mean ± SEM) from 3 experiments with a total of 17–18 mice per group (b,e–l) and pooled data from 2 experiments with 8–12 mice per group (c,d) are shown.
