Figure 8
From: Functional Transcriptome Analysis in ARSACS KO Cell Model Reveals a Role of Sacsin in Autophagy
Mitophagic flux is altered in sacsin KO cells. (A–D) Immunofluorescence images of the mitophagy-related protein Parkin (red) and LC3 (green) in WT and KO cells under normal conditions (A,C) or upon FCCP treatment (B,D). There was no fusion between damaged mitochondria and autophagosomes in sacsin KO cells. Co-localization of Parkin with LC3 (yellow) is arrow indicated. (E–H) Co-staining of PINK1 (green) and TOM20 (in red) in WT and KO cells under normal conditions (E,G) or FCCP treatment (F,H) indicated a reduced localization of PINK1 on the outer mitochondrial membrane in KO cells after FCCP treatment. DAPI (in blue) was used as nuclear stain. Scale bar = 10 µm.
