Figure 2

Elevated IOP following microbead injection decreases anterograde transport in SMs. (a) Schematic showing microbead and CTB injection paradigm. Left eyes received saline and CTB-488 (green) while right eyes received microbeads and CTB-594 (red). (b) Whole-mounted retinas from saline- and microbead-injected retinas showing CTB (green or red) uptake by RGC somas (arrowheads) and transport in axons (dotted lines). Phosphorylated neurofilament heavy (pNFH, blue) expression is shown in RGC somas and axons (c,d) Coronal section of a LGN from a naïve SM or an experimental SM. CTB transported from the left eye (LE, green) and the right eye (RE, red) is shown. (e) Coronal sections of a SC from a naïve or an experimental SM. (f) Bar graph showing percent intact transport to the LGN and SC from saline- and microbead-injected eyes. *p < 0.0006; n indicated in bars. Data are expressed as mean ± SEM. Statistical comparisons were made using two-sided t-tests.