Figure 3

Elevated IOP decreases RGC axons in SM optic nerve. (a) Representative images of SM optic nerves following saline or microbead injection. (b) Optic nerve images from microbead-injected eyes showing degenerating axon profiles surrounded by intact axons (arrowheads, left) and severe degeneration where few intact axons remain (right). (c–e) Scatter plots showing mean area of optic nerves, mean RGC axon number, and mean degenerating axon profiles from saline- and microbead-injected SM eyes. Black lines indicate mean, red bars indicate SEM. *p = 0.038 (c), p = 0.042 (d), and p = 0.007 (e); n = 7 for each group. Data are expressed as mean ± SEM. Statistical comparisons were made using two-sided t-tests.